<scp>RNA</scp> interference‐mediated silencing of hypoxia‐inducible factor 1 signaling pathway genes enhanced the hypoxia sensitivity in the brown‐legged grain mite, <scp> <i>Aleuroglyphus ovatus</i> </scp>

Mortality acceleration, developmental delays, and pathway co-regulation under hypoxia expose vulnerabilities of A. ovatus exploitable for pest management. Data confirm that combining atmospheric controls with single-gene RNAi targeting any of these three genes enhances lethality. As a systematic dissection of the HIF1 signaling pathway's hypoxic response in arthropods-a rarely explored area-this work established an interdisciplinary strategy integrating ecological interventions and molecular silencing, providing a theoretical basis for sustainable pest control. © 2025 Society of Chemical Industry.