Orodispersible film based on hyaluronic acid and morin for dental biofilm control

Orodispersible films (ODFs) are an innovative oral drug delivery method benefiting pediatric, geriatric, and non-compliant patients. They are portable, easy to swallow, and enhance bioavailability. Hyaluronic acid (HA) stands out among hydrophilic polymers for oral delivery of antimicrobial agents. This study evaluated the physicochemical properties, <i>in vitro</i> release profile, and antimicrobial/antibiofilm activity of HA-based ODFs combined with the flavonoid morin, known for its antimicrobial properties. Antimicrobial activity and microbial viability were assessed <i>via</i> biomass quantification. The films were thin (12-27 µm), flexible, homogeneous, and mechanically resistant. A burst release of morin was observed, reaching complete release at 210 min. Cytotoxicity analysis confirmed the non-toxic profile, showing cell viability. HA-morin films significantly reduced <i>Streptococcus mutans</i> biofilm mass, viability, and acidogenicity compared to the controls. Findings confirmed the non-toxic, and their significant antibiofilm activity against <i>S. mutans</i>. This innovative mucoadhesive system has potential for managing dental diseases and oral drug delivery.