Figure 4 from SALL4 Is Required for YAP1-Dependent Malignant and Regenerative Hepatocyte-to-Cholangiocyte Reprogramming

<p><i>Bmi1</i> modulation phenocopied <i>Sall4</i> modulation in <i>AY</i>-driven HC-to-CCA tumorigenesis. <b>A,</b> qRT-PCR analysis showing <i>Bmi1</i> expression in <i>AY-Sall4</i> compared with <i>AY-GFP</i>. <b>B,</b> Schematic experimental design. <b>C</b> and <b>H,</b> Representative macroscopic images of livers harvested from indicated groups. <b>D,</b> LW/BW ratio comparison (<i>n</i> = 3–5 per group). <b>E</b> and <b>I,</b> IHC analysis for indicated markers (HA-tag, HNF4A, Ki-67, panCK, and V5-tag) in experimental groups. <b>F</b> and <b>J,</b> Quantification of Ki-67<sup>+</sup> proliferating cells (%) and lipid droplets area per field (%) comparing <i>Bmi1 KO</i><sup><i>CCAΔ</i></sup> and <i>AY-Bmi1</i> tumors. <b>G,</b> qRT-PCR analysis showing <i>Bmi1</i> expression in <i>AY-Bmi1</i> compared with <i>AY-GFP</i>. Data are presented as the mean ± SD; Student <i>t</i> test (*, <i>P</i> < 0.05; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001). Scale bars, (<b>E</b> and <b>I</b>) 2 mm (overview), 200 μm (magnified views).</p>