Ultrafast WGS protocol v1

Ultra-Fast Whole Genome Sequencing (UF-WGS) offers rapid, comprehensive molecular profiling in paediatric haematology-oncology, enabling critical improvements in diagnosis and patient management. In a prospective study of 54 children with suspected or confirmed cancer, UF-WGS reduced turnaround time from 37 to 3 days versus standard NHS practice and captured 95% of all clinically actionable variants found by conventional methods. UF-WGS also identified 19 additional actionable variants, leading to demonstrable improvements in care for 51% of prospective patients, including avoidance of over-medicalisation and support for timely precision therapy. UF-WGS was feasible across diverse tumour types and sample sources, with additional technical advantages in variant detection and workflow simplicity. These results support UF-WGS as a transformative single-test approach in paediatric oncology, facilitating safer, more rapid allocation of targeted treatment and precise risk stratification.