First Report of Vairimorpha (Nosema) ceranae in Apiaries of Campeche, Mexico: Molecular Detection and Prevalence
Nosemosis, caused by microsporidians of the genus <i>Vairimorpha</i>, is one of the most significant diseases affecting the honey bee <i>Apis mellifera</i> L. (Apidae: Hymenoptera), with negative impacts on its health and productivity. This study aimed to assess the presence of <i>Vairimorpha ceranae</i> in apiaries from the state of Campeche, Mexico. Honey bee samples were collected from 79 colonies across 29 apiaries, and DNA was extracted for analysis. Detection of the microsporidian parasite was achieved through amplification of the 16S small subunit ribosomal RNA gene by PCR and PAGE, revealing a high prevalence of <i>Vairimorpha ceranae</i>. The results represent the first confirmed record of <i>Vairimorpha ceranae</i> in this region, with widespread infection detected among sampled colonies. This finding is crucial for identifying the frequency and geographic distribution of this parasite in Mexico and will support the development of targeted management and control strategies adapted to local conditions, ultimately contributing to the conservation and health of honey bee populations in Campeche.
Authors
- Fulgencio Alatorre‐Cobos (ORCID: https://orcid.org/0000-0001-8114-6180)
- Castellanos-Zacarías Carlos
- Henry Loeza-Concha (ORCID: https://orcid.org/0000-0001-7686-5113)
- Álvaro Domínguez-Rebolledo (ORCID: https://orcid.org/0000-0002-1444-3844)
- Carlos Granados-Echegoyen (ORCID: https://orcid.org/0000-0003-2507-0729)
- Alfredo Sánchez‐Villarreal (ORCID: https://orcid.org/0000-0002-3702-6736)
- Clemente Lemus Flores (ORCID: https://orcid.org/0000-0002-5120-6805)
- Cláudio Roberto Marquetto Maurício (ORCID: https://orcid.org/0000-0003-2783-3988)
- José Octavio Macías-Macías (ORCID: https://orcid.org/0000-0002-8369-0523)
- Dany Alejandro Dzib-Cauich
- Alberto Santillán-Fernández (ORCID: https://orcid.org/0000-0001-9465-1979)
- Rodríguez-Salinas Arturo (ORCID: https://orcid.org/0000-0001-9661-1209)
- Us-Camas Rosa
Institutions
- Instituto Tecnológico de Oaxaca (MX)
- Universidad de Guadalajara (MX)
- Universidad Autónoma de Nayarit (MX)
- Autonomous University of Campeche (MX)
- Instituto Tecnológico Superior de Xalapa (MX)
- Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (MX)
- Tecnológico Nacional de México (MX)
- Colegio de Postgraduados (MX)
- Secretaría de Investigación, Innovación y Educación Superior (MX)
- Instituto Tecnológico de León (MX)
- Instituto Politécnico Nacional (MX)
Publication Details
- Journal
- Insects
- Published
- 2025-09-25
- DOI
- https://doi.org/10.3390/insects16100996
- Primary Topic
- Insect and Pesticide Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00