Carbon source utilization regulates biofilm formation and plant-beneficial interactions of Pseudomonas ogarae F113
Root exudate determines the gene expression of root associated bacteria, but the underlying mechanisms are poorly understood. We tested the hypothesis that carbon sources within root exudates regulate interactions of beneficial bacteria with the plant via carbon catabolite repression (CCR). Mutants in CCR genes were constructed in <i>Pseudomonas ogarae</i> F113, and rhizospheric traits were studied. <i>P. ogarae</i> F113 displays reverse CCR. The <i>Δcrc</i> mutant produced more biofilm than the wild-type strain, and its swimming was carbon source dependent. On roots, the bacterial expression of <i>nirS, crcY,</i> and <i>crcZ</i> was higher in the <i>Δcrc</i> mutant. Auxin expression (but not jasmonate signaling) in <i>Arabidopsis thaliana</i> was reduced upon inoculation by <i>Δcrc</i> or <i>ΔcbrB</i> mutants when compared with the wild-type strain. Our findings show that reverse CCR regulates the communication between <i>P. ogarae</i> F113 and <i>A. thaliana</i>, which sheds further light on the significance of root exudates for the functioning of plant-beneficial bacteria on roots.
Authors
- Théophile Franzino
- Judit Szécsi (ORCID: https://orcid.org/0000-0002-1630-803X)
- Mohammed Bendahmane (ORCID: https://orcid.org/0000-0003-1661-1060)
- Florian Mermillod‐Blondin (ORCID: https://orcid.org/0000-0001-5685-2892)
- Feth el Zahar Haichar (ORCID: https://orcid.org/0000-0003-3687-0529)
- Yvan Moënne‐Loccoz (ORCID: https://orcid.org/0000-0002-9817-1953)
- Hasna Boubakri (ORCID: https://orcid.org/0000-0001-5911-6153)
- Lisa Merlin
- Amandine M'Sakni
- Michel Droux
Institutions
- Université Claude Bernard Lyon 1 (FR)
- École Normale Supérieure de Lyon (FR)
- Centre National de la Recherche Scientifique (FR)
- Institut Universitaire de France (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- VetAgro Sup (FR)
- Laboratoire de Microbiologie, Adaptation et Pathogénie (FR)
- Laboratoire Reproduction et Développement des Plantes (FR)
Publication Details
- Journal
- iScience
- Published
- 2025-09-25
- DOI
- https://doi.org/10.1016/j.isci.2025.113639
- Citations
- 1
- Primary Topic
- Plant-Microbe Interactions and Immunity
- Type
- article
- Field-Weighted Citation Impact
- 1.75
Funders
- Indian National Science Academy
- Agence Nationale de la Recherche
- Institut National des Sciences Appliquées de Lyon
- École Normale Supérieure de Lyon
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement