Potential markers of obesity linked to the gut microbiome

Objective: to study the features of the content of some types of microorganisms in the colon in obese patients and healthy individuals with normal body weight using metagenomic sequencing. Materials and methods: a total of 265 people (44 men and 221 women, average age 47.1±4.8 years) were examined. Two clinical groups were formed: healthy individuals with normal body weight (n=129) and obese patients (n=136). The composition of the intestinal microbiome was assessed using metagenomic analysis. DNA was isolated from fecal samples and sequencing of the v3-v4 variable region of the 16S rRNA gene was performed. Results: statistically significant (p<0.05) differences in the quantitative and qualitative parameters of some types of microorganisms in the colon of healthy individuals without obesity and in obese patients were revealed. The number of P. copri was increased in obese patients. and decreased F. prausnitzii , B. adolescentis and B. longum . Conclusions: the obtained results demonstrated certain species differences in the intestinal microbiome in healthy individuals and in obese patients.