In vitro rejeneration potential of Matricaria recutita L. capitulum explant

Purpose: In this study, the in vitro regeneration potential of Matricaria recutita L., a medicinal and aromatic plant, was investigated Method: Six different sterilization processes were tested to determine the sterilization protocol of capitulum explants isolated from plants grown under field conditions. For regeneration studies, plant growth regulators BAP (0.5-1-2 mg/L) and NAA (0-0.5-1-2 mg/L); KIN (0.5-1-2 mg/L) and 2.4-D (0-0.5 mg/L) were applied to capitulum explants at 24 different concentrations and combinations. Findings: In the experiments carried out for sterilization purposes, the best results were obtained in 70% ethanol for 3 minutes, in 30% NaOCl for 10 minutes and in 1% AgNO3 for 20 minutes. The best shoot lengths were determined as 3.02 and 3.00 cm in MS nutrient medium containing 0.5 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L BAP+2 mg/L NAA, respectively; the best shoot number per explant was determined as 23.91 and 18.29 in MS nutrient medium containing 0.5 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L BAP+2 mg/L NAA plant growth regulators, respectively. Conclusion: According to the results obtained from in vitro regeneration studies, in terms of the applied plant growth regulator combinations, BAP and NAA gave more successful results than KIN and 2.4-D applications