Evaluation of Colistin Susceptibility in ICU Isolates of Carbapenemase-Producing Klebsiella pneumoniae

Introduction: The emergence of colistin-resistant carbapenem-resistant Klebsiella pneumoniae poses a significant threat in intensive care units, reducing available treatment options and complicating patient management. Monitoring its occurrence and distribution is essential for guiding infection control and antimicrobial stewardship strategies. Materials and Methods: A total of 242 carbapenem-resistant Klebsiella pneumoniae isolates were collected from intensive care unit patients. Initial screening for colistin resistance was conducted using VITEK 2, followed by confirmation with the reference Broth Microdilution method. Data on patient demographics, specimen type, and ICU source were recorded and analyzed. Results: Out of 242 carbapenem-resistant Klebsiella pneumoniae isolates, 18 (7.4%) were identified as colistin-resistant by VITEK 2, and 13 (5.4%) were confirmed by Broth Microdilution. colistin resistant Klebsiella pneumonaie isolates were predominantly from male patients (9/13, 69.2%), with most patients in the 41?60 years age group. Department-wise, the majority were recovered from the Neuro intensive care unit (8/13, 61.5%). Respiratory specimens were the most common source (8/13, 61.5%), including endotracheal aspirates (5/13, 38.5%) and sputum (3/13, 23.1%). Conclusion: The detection of colistin resistant Klebsiella pneumonaie in intensive care unit settings highlights the urgent need for accurate diagnostic methods, ongoing surveillance, and robust infection control practices. Implementation of antimicrobial stewardship programs is critical to curb the spread of these highly resistant pathogens.