Role of Genotype Activities of COMT Gene in Male Schizophrenia Patients, Tumkur District, South Karnataka

Introduction:Schizophrenia (SCZ) is a neuropsychiatric & multi-symptomatic disease. Catechol-O-Methyltransferase (COMT) gene is candidate gene associate with SCZ. COMT gene [Catecho-O-methyltrasnferase] encodes enzymatic activity, which interlink with various metabolic pathways like steroidal hormonal pathways. COMT gene exits multiple polymorphic forms, genotype activity and their affects’ associated with phenotypes & sex hormonal production. Aim: The study was designed to evaluate the genotype activity associate with male SCZ patients in Tumkur district, South Karnataka. Objectives: To study of COMT genotype in exon: 3 & exon: 4. To study of genotype activity on sex-hormonal biochemical serum marker. Subjects & methods:The study was performed on 80 SCZ patients & 80 healthy groups between 16-70 years. We procured blood samples for DNA isolation. Thermo cycle reaction [PCR] was used to amplify the COMT gene, 1% Agarose gel electrophorisis, Sanger sequencing. For hormonal analysis was done by CLIA method. Results: This study analyzed demographic information from male schizophrenia patients. The mutational genotypes G/A [Val/Met], C/T [H62H] & G/C [Ala/Ala] associated with symptoms. However, G/A [Val/Met] & C/T [H62H] are strongly associated with serum E2 & Testosterone level are found [P<0.01] statistically significant. Conclusions:The genotype activity of the COMT gene distinguishes from genotype to genotype. In this study results concluded, that the genotype of COMT genes G/A [Val/Met] & C/T [H62H] are strongly associated with phenotypes and serum E2 & Testosterone level in schizophrenia patients in Tumkur district. Moreover, we focus on molecular docking and epigenetic modification.