The impact of probiotic administration in vivo on peritoneal mouse macrophages infected by Leishmania amazonensis ex vivo
PB8 or Bifilac administration significantly reduced (p < 0.05) ex vivo infection of PMMs from male mice by 27% and 12%, respectively. No gender-dependent effect of probiotics was observed. No improved antiparasitic activity of 1 µM miltefosine or N 6-methyltubercidin was observed in probiotic-treated PMMs. Ex vivo Leishmania infection stimulated tumour necrosis factor (TNF), MCP-1, and interleukin-6 (IL-6) production by PMMs (p < 0.05). A trend of increase was recorded with elevated levels of TNF and IL-6 in PB8-treated male groups (around 43 and 52%, respectively) but were not statistically significant. Collectively, probiotic treatment of mice influences Leishmania infection in PMMs. Clinical applications in leishmaniasis warrant further studies.
Authors
- Ludmila Ferreira de Almeida Fiuza (ORCID: https://orcid.org/0000-0001-6707-8556)
- Marcelo Meuser-Batista (ORCID: https://orcid.org/0000-0002-2399-9967)
- Serge Van Calenbergh (ORCID: https://orcid.org/0000-0002-4201-1264)
- Maria de Nazaré Correia Soeiro (ORCID: https://orcid.org/0000-0003-0078-6106)
- Guy Caljon (ORCID: https://orcid.org/0000-0002-4870-3202)
- Cynthia Machado Cascabulho (ORCID: https://orcid.org/0000-0001-6432-6657)
- Raquel Azevedo (ORCID: https://orcid.org/0000-0003-2896-7224)
- E. Van de Velde
- Lauren Van den Broeck
Institutions
- University of Antwerp (BE)
- Provincial Institute for Hygiene (BE)
- Health Level Seven International (BE)
- Fundação Oswaldo Cruz (BR)
Publication Details
- Journal
- Memórias do Instituto Oswaldo Cruz
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1590/0074-02760250014
- Primary Topic
- Child Nutrition and Feeding Issues
- Type
- article
- Field-Weighted Citation Impact
- 0.00