Multicenter comparison of LC-MS/MS, radioimmunoassay, and ELISA for assessment of salivary cortisol and testosterone
LC-MS/MS was the best-performing method for both C and T across all criteria examined. RIA, despite showing slightly higher variance, can still be considered a reliable analytical technique as it met most of the set criteria for C and T. On the other hand, ELISA overestimated values, especially at low T levels. Therefore, caution should be exercised when selecting an appropriate method for the specific need.
Authors
- Justin M. Carré (ORCID: https://orcid.org/0000-0002-8967-9288)
- Manfred Rauh (ORCID: https://orcid.org/0000-0002-4852-7157)
- Oliver C. Schultheiss (ORCID: https://orcid.org/0000-0002-7892-5228)
- Ashley L. Marcellus
- Gelena Dlugash (ORCID: https://orcid.org/0000-0003-1684-0760)
- Susan Plachecki
Institutions
- Nipissing University (CA)
- Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
- Technische Universität Dresden (DE)
Publication Details
- Journal
- Psychoneuroendocrinology
- Published
- 2025-09-24
- DOI
- https://doi.org/10.1016/j.psyneuen.2025.107618
- Primary Topic
- Stress Responses and Cortisol
- Type
- article
- Field-Weighted Citation Impact
- 0.00