Rapid and visual on-site detection system for Epizootic Hemorrhagic Disease Virus based on a combination of CRISPR-Cas12a and RT-ERA

1. First development of a RT-ERA/CRISPR-Cas12a platform for rapid and visual detection of EHDV, enabling on-site diagnosis within 1 hour without specialized equipment. 2. High sensitivity and broad serotype coverage: The assay detects as low as 1.7 × 10 1 copies reaction -1 and recognizes 8 EHDV serotypes (1, 2, 4–8, and 10), overcoming the limitation of serotype-specific antisera required in traditional methods. 3. Integration with HUDSON sample processing allows direct detection from crude samples without RNA extraction, making it suitable for field use in resource-limited settings and supporting point-of-care testing in ruminant populations.