Anti-hyperuricemic triterpene saponins from the roots of <i>Achyranthes bidentata</i>
Three new triterpene saponins (<b>1-3</b>) were obtained from <i>Achyranthes bidentata</i>, which were further assayed for the anti-hyperuricemic activities. Their structures were determined through HRESI-MS, NMR data and chemical method. As for the anti-hyperuricemic evaluation, <b>1-3</b> were evaluated against the adenosine/xanthine oxidase induced HK-2 cell model. Compound <b>1</b> showed the most effective activity at 50.0 μM, <b>2</b> and <b>3</b> showed moderate anti-hyperuricemic activities at 50.0 μM and 12.5 μM, respectively, with <b>1</b> and <b>2</b> concentration-dependent, while <b>3</b> on the contrary. Western blot analysis was carried out to investigate the mechanism of <b>1</b>, demonstrating that <b>1</b> reduced uric acid levels partly by regulating the renal urate transporters (OAT1, URAT1 and GLUT9).
Authors
- Lei Zhao (ORCID: https://orcid.org/0000-0003-0813-6140)
- Tao Yue
- G.A.S. Premakumara (ORCID: https://orcid.org/0000-0001-8761-6614)
- Jinqian Yu (ORCID: https://orcid.org/0000-0002-7709-4610)
- Tong Niu (ORCID: https://orcid.org/0009-0007-8376-7301)
- Xiao Dan Wang (ORCID: https://orcid.org/0000-0003-4236-2483)
Institutions
- Qingdao University of Science and Technology (CN)
- Qilu University of Technology (CN)
- Shandong Academy of Sciences (CN)
- Shandong University of Science and Technology (CN)
- Defense Acquisition University (US)
- University of Colombo (LK)
Publication Details
- Journal
- Natural Product Research
- Published
- 2025-09-24
- DOI
- https://doi.org/10.1080/14786419.2025.2559775
- Primary Topic
- Natural product bioactivities and synthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00