Enzymatic synthesis of stable isotope-labeled SAM for screening hMAT2A inhibitors by using matrix-assisted laser desorption/ionization mass spectrometry
A high-throughput method was developed for screening human MAT2A (hMAT2A) inhibitors by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS). The stable isotope deuterium-labeled S-adenosylmethionine (SAM-D<sub>3</sub>) was enzymatically synthesized by hMAT2A using L-Met-D<sub>3</sub> and ATP as substrates and used as the internal standard for the enzyme activity assay, which could significantly improve the quantitative accuracy of high-throughput screening.
Authors
- Lingna Zheng (ORCID: https://orcid.org/0000-0001-7480-7331)
- H. Lu (ORCID: https://orcid.org/0000-0002-8879-1446)
- Q. J. Li (ORCID: https://orcid.org/0009-0003-6921-7291)
- Xiang Gao (ORCID: https://orcid.org/0000-0002-7496-158X)
- Qing Yang (ORCID: https://orcid.org/0000-0002-7529-9065)
- Chunjing Chen (ORCID: https://orcid.org/0009-0009-1270-6530)
- Yixian Liu (ORCID: https://orcid.org/0009-0007-4087-7885)
Institutions
- Fujian Medical University (CN)
- Xiamen University (CN)
- Union Hospital (CN)
Publication Details
- Journal
- The Analyst
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5an00705d
- Primary Topic
- Peptidase Inhibition and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00