Preconcentration and Seperation of Indigo Carmine (E132) in Food Samples by Dispersive Solid Phase Microextraction Method

In this study, a microextraction technique was produced using Fe3O4@XAD-7 nanoparticle for the preconcentration and separation of Indigo Carmine (E132) in some food samples prior to spectrophotometric analysis. Firstly, optimum conditions of parameters of developed method such as pH, amount of Fe3O4@XAD-7 nanoparticle, type of desorption solvent, vortex time, sample volume were determined. Under the optimum conditions, the obtained results, developed solid phase microextraction method possessed excellent linearity with a correlation coefficient (R2) of 0.999 in the concentration range of 8–250 μgL̀-1 and detection limit are 2.1 μgL-1. Relative standard deviation % (RSD%) of the method was calculated as 2.3%. Enhancement factor (EF) and preconcentration factor (PF) of the developed method were calculated to be 85 and 71, respectively. The developed method was applied to real food samples and high recoveries were obtained in the range of 98-105%.