A novel ZIKV-targeted scRNA-seq method for precise quantification of ZIKV RNA
This study marks the first use of a scRNA-seq method tailored for ZIKV, allowing accurate measurement of ZIKV RNA in individual cells and identification of critical susceptible cell types. A comparative analysis with the 10× Genomics scRNA-seq method highlighted the advantages of ZIKV-targeted scRNA-seq in terms of accuracy and practicality, particularly its superior ability to capture exogenous cells. Beyond ZIKV, this method also helps establish precise quantification of viral RNA at the single-cell level for other viruses by designing target-specific beads based on conserved regions of the viral genome. This advancement is set to greatly enhance studying pathogenesis of ZIKV infection and then significantly contribute to improve prevention and research in therapeutics and vaccines.
Authors
- Dongying Fan (ORCID: https://orcid.org/0009-0002-1634-7431)
- Libo Liu (ORCID: https://orcid.org/0000-0001-5773-0184)
- Yang Zhou
- Yuxuan Liu (ORCID: https://orcid.org/0009-0002-9684-6416)
- Peigang Wang (ORCID: https://orcid.org/0000-0001-6045-2007)
- Kevin Lin (ORCID: https://orcid.org/0000-0002-1236-9847)
- Jing An (ORCID: https://orcid.org/0000-0002-2567-0380)
- Wei Yang (ORCID: https://orcid.org/0000-0003-1973-5579)
- Yisong Wang (ORCID: https://orcid.org/0000-0002-1609-2523)
- Chunlong Zhong (ORCID: https://orcid.org/0000-0002-0605-7273)
- Yanhua Wu (ORCID: https://orcid.org/0009-0001-1579-7599)
Institutions
- Guiyang Medical University (CN)
- Capital Medical University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Affiliated Hospital of Guizhou Medical University (CN)
- Institute of Laboratory Animal Science (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2025-09-24
- DOI
- https://doi.org/10.1128/jvi.01114-25
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 0.00