18S/28S rDNA metabarcoding identifies Cryptosporidium parvum and Blastocystis ST1 as the predominant intestinal protozoa in hospital patients from Changchun, Northeast China
Metabarcoding of rDNA amplicons provides a feasible snapshot of human intestinal‑parasite communities in Northeast China, revealing C. parvum IIdA19G1 as an emerging zoonotic threat and highlighting ongoing food‑borne trematodiasis. However, the overwhelming amplification of fungal templates and inter‑primer bias call for primer redesign and complementary diagnostics before routine clinical adoption.
Authors
- Zhanpeng Shi
- Guan Zhu (ORCID: https://orcid.org/0000-0003-3888-0659)
- Junhong Chen (ORCID: https://orcid.org/0000-0002-1578-4667)
- Cunmin Wang
- Yueyang Yan
- Jigang Yin (ORCID: https://orcid.org/0000-0002-5819-9206)
- Jixue Zhao (ORCID: https://orcid.org/0000-0003-3998-2729)
- Yijia Xu (ORCID: https://orcid.org/0009-0000-9501-3012)
Institutions
- Jilin University (CN)
- First Hospital of Jilin University (CN)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2025-09-24
- DOI
- https://doi.org/10.1186/s13071-025-07043-z
- Citations
- 1
- Primary Topic
- Parasitic Infections and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 2.05
Funders
- People's Government of Jilin Province