Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

This protocol produces multiplexed amplicon libraries from DNA extracted from general and selective bacterial enrichments using Nanopore's Ligation sequencing kit, suitable for sequencing on Oxford Nanopore Technologies‱ (ONT) MinION/Gridion systems using ONT V14 chemistry (SQK-NBD114-96).

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Publication Details

Published
2025-06-16
DOI
https://doi.org/10.17504/protocols.io.81wgbkjqqgpk/v2
Primary Topic
Microbial Community Ecology and Physiology
Type
article
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article

Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

Padmini Ramachandran, Christopher Duda
Microbial Community Ecology and Physiology
article

Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

Padmini Ramachandran, Christopher Duda
article en

Abstract

This protocol produces multiplexed amplicon libraries from DNA extracted from general and selective bacterial enrichments using Nanopore's Ligation sequencing kit, suitable for sequencing on Oxford Nanopore Technologies‱ (ONT) MinION/Gridion systems using ONT V14 chemistry (SQK-NBD114-96).

Openalex Percentile: Top 75%
Microbial Community Ecology and Physiology
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Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2 — Padmini Ramachandran, Christopher Duda · (2025) | TGRS Research Map | TGRS