Isolation, Production and Optimization of Gelatinase from Organic Waste

The OWS4 isolate exhibited the highest gelatinase activity and was tentatively identified as <i>Serratia marcescens</i> based on morphological and biochemical assessments. Additionally, various physiological parameters were examined, including pH and temperature. The gelatinase exhibited peak activity at a pH of 6.6, with the optimal temperature for gelatinase activity determined to be 20°C. The Bacterial isolated OWS4 was a good source of gelatinase as it showed high gelatinase activity. It showed a largest clear zone on gelatin agar medium plate of 1.9 mm in diameter. The isolate OWS4 produced amount of gelatinase enzyme 0.77/per 50 mL medium unit after the incubation of 24 to 48 hrs.