Proteomic profiling identifies miR-423-5p as a modulator of oncogenic metabolism in HCC
miR-423-5p acts as a tumor suppressor in HCC by targeting key nodes of pro-tumorigenic signalling. miR-423-5p significantly altered metabolic pathways, including purine/pyrimidine metabolism and gluconeogenesis. Seven miR-423-5p targets correlate with poor prognosis in TCGA-LIHC patients and are downregulated in miR-423-5p overexpressing HCC cells. miR-423-5p over-expression induces a significant downregulation of MCM7, DVL3, IMPDH1, SPEE in HCC cell models. miR-423-5p limits tumor metabolic plasticity, suggesting therapeutic potential.
Authors
- Elisabetta A. M. Verderio (ORCID: https://orcid.org/0000-0001-9153-8997)
- Madhura S. Tathode
- Rossella Sperlongano
- Amalia Luce (ORCID: https://orcid.org/0000-0003-0372-6870)
- Luigi Mele (ORCID: https://orcid.org/0000-0002-6008-0802)
- Gabriella Misso (ORCID: https://orcid.org/0000-0002-7787-2531)
- Silvia Zappavigna
- David J. Boocock (ORCID: https://orcid.org/0000-0002-7333-3549)
- Maria Preziosa Romano (ORCID: https://orcid.org/0000-0002-4469-2526)
- Michele Caraglia (ORCID: https://orcid.org/0000-0003-2408-6091)
- Ines Simeone
- Marco Bocchetti (ORCID: https://orcid.org/0000-0002-2143-6669)
- Clare Coveney (ORCID: https://orcid.org/0000-0001-7047-6408)
- Alessia Maria Cossu
- Giovanni Vitale (ORCID: https://orcid.org/0000-0003-2478-683X)
- Maria Roberta De Iesu
Institutions
- University of Sannio (IT)
- University of Milan (IT)
- University of Campania "Luigi Vanvitelli" (IT)
- University of Basilicata (IT)
- Biogem (IT)
- University of Campania "Luigi Vanvitelli" (IT)
- IRCCS Istituto Auxologico Italiano (IT)
- Istituti di Ricovero e Cura a Carattere Scientifico (IT)
- IRCCS Humanitas Research Hospital (IT)
- Link Campus University (IT)
- Nottingham Trent University (GB)
- University of Bologna (IT)
Publication Details
- Journal
- Journal of Translational Medicine
- Published
- 2025-09-24
- DOI
- https://doi.org/10.1186/s12967-025-07039-4
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Eye Institute