Metabolite-enhanced normothermic machine perfusion improves kidney transplant viability
Normothermic machine perfusion (NMP) has become a valuable tool to expand the pool of transplantable organs. However, the application of NMP to kidneys presents substantial challenges, mostly due to high variability in the composition of currently used perfusion solutions. Here, we provide a multimodal cross-species cellular atlas of kidney injury associated with NMP using a literature-based consensus buffer. This resource provided a systematic framework that was used to develop a metabolite-enhanced perfusion solution, which protected renal proximal tubular cells, improving cellular viability and transplantation outcomes across species, including human kidneys.
Authors
- Florian Grahammer (ORCID: https://orcid.org/0000-0002-7851-754X)
- Sonja Hänzelmann (ORCID: https://orcid.org/0000-0003-4953-0101)
- Ilka Edenhofer
- Sydney E. Gies (ORCID: https://orcid.org/0009-0002-4264-7083)
- Marina Zimmermann (ORCID: https://orcid.org/0000-0002-2666-5997)
- Jan Czogalla (ORCID: https://orcid.org/0000-0003-1888-3369)
- Stefan Bonn (ORCID: https://orcid.org/0000-0003-4366-5662)
- Silvia Chilla
- Malte Kuehl (ORCID: https://orcid.org/0000-0003-4167-2498)
- Gisela Ambagtsheer (ORCID: https://orcid.org/0009-0004-5354-3639)
- Sarah Bouari (ORCID: https://orcid.org/0000-0003-1878-5427)
- Fabian Haas (ORCID: https://orcid.org/0000-0001-5440-1035)
- Victor G. Puelles (ORCID: https://orcid.org/0000-0002-7735-5462)
- Yusuke Okabayashi (ORCID: https://orcid.org/0000-0001-7150-6947)
- Smilla Hofmann
- Anne K. Mühlig
- Fabian Hausmann (ORCID: https://orcid.org/0000-0001-6110-5824)
- Maja T. Lindenmeyer (ORCID: https://orcid.org/0000-0003-4162-9107)
- Yitian Fang (ORCID: https://orcid.org/0000-0002-5523-9794)
- Robert C. Minnee (ORCID: https://orcid.org/0000-0002-9494-3717)
- Martin J. Hoogduijn (ORCID: https://orcid.org/0000-0002-0217-8254)
- Tobias B. Huber (ORCID: https://orcid.org/0000-0001-7175-5062)
- Oliver Kretz (ORCID: https://orcid.org/0000-0003-1152-5956)
- Rossana Franzin (ORCID: https://orcid.org/0000-0001-7907-7614)
- Simon Lagies (ORCID: https://orcid.org/0000-0002-5245-1781)
- Bernd Kammerer (ORCID: https://orcid.org/0000-0002-4631-8853)
- R. de Bruin
- Nico Kaiser (ORCID: https://orcid.org/0009-0006-6054-2831)
- Takashi Yokoo
- Milagros N. Wong (ORCID: https://orcid.org/0009-0008-6372-7094)
- Dominik Kylies
- Loreto Gesualdo
- Sabrina Christiansen
- Niklas Sabra
Institutions
- Jikei University School of Medicine (JP)
- Universität Hamburg (DE)
- University of Freiburg (DE)
- Aarhus University (DK)
- University Medical Center Freiburg (DE)
- Erasmus MC (NL)
- Aarhus University Hospital (DK)
- Core Competence (US)
- University Medical Center Hamburg-Eppendorf (DE)
- Kinderkrebs-Zentrum Hamburg (DE)
- Hamburg Institut (Germany) (DE)
- Institute of Medical Microbiology and Hygiene (DE)
- Erasmus MC Cancer Institute (NL)
- University of Bari Aldo Moro (IT)
Publication Details
- Journal
- JCI Insight
- Published
- 2025-09-22
- DOI
- https://doi.org/10.1172/jci.insight.190185
- Primary Topic
- Renal Transplantation Outcomes and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00