Improving the diagnostic performance for prior COVID-19 with T-SPOT, an interferon-gamma release assay
Frozen PBMCs were useful for performing T-SPOT assays. The combination of T-SPOT assays targeting nucleocapsid protein and antibody tests improved the diagnosis of past SARS-CoV-2 infections in vaccinated participants. These findings suggest that integrating cellular and humoral immunity assays can facilitate COVID-19 prevalence studies.
Authors
- Takahisa Kawaguchi (ORCID: https://orcid.org/0000-0001-7781-7404)
- Kazuya Setoh (ORCID: https://orcid.org/0000-0001-9827-1455)
- Miki Nagao (ORCID: https://orcid.org/0000-0002-8886-6145)
- Koh Shinohara (ORCID: https://orcid.org/0000-0001-8934-0375)
- Yasuhiro Tsuchido (ORCID: https://orcid.org/0000-0003-1306-3652)
- Fumihiko Matsuda
- Michael White (ORCID: https://orcid.org/0000-0002-7472-4138)
- Masaki Yamamoto (ORCID: https://orcid.org/0000-0002-0288-7990)
- Yusuke Tsuda
- Yasufumi Matsumura
Institutions
- Inserm (FR)
- Institut Pasteur (FR)
- Université Paris Cité (FR)
- Kyoto University (JP)
- Kyoto Prefectural University of Medicine (JP)
- Kyoto International University (JP)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-09-24
- DOI
- https://doi.org/10.3389/fmicb.2025.1675605
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00