The first haplotype-resolved genome assembly of Prunus s.l. subgenus Laurocerasus (Prunus spinulosa)

<i>Prunus spinulosa</i> (2n = 4x = 32) is an evergreen species of significant medicinal usage and ecological value. However, the lacking of a high-quality genome of <i>P. spinulosa</i> has obstructed further exploration of its ecological study and phylogenetic relationship of <i>Prunus</i>. In this study, we present the first haplotype-resolved genome assembly of <i>Prunus</i> s.l. subgenus <i>Laurocerasus</i>, the tetraploid genome of <i>P. spinulosa</i> was phased into 32 pseudochromosomes with 4 haplotypes, the genome size of each haplotype ranged from 249.82 Mb to 259.69 Mb, and N50 fluctuated from 31.35 Mb to 33.25 Mb, the protein-coding genes vary from 21,272 to 22,668. Different evaluation methods showed that the <i>P. spinulosa</i> genome assembly has high quality of completeness, continuity and accuracy. Being the first complete genome of <i>P. spinulosa</i>, it provides a valuable genetic resource for the <i>Prunus</i> tetraploid species database and supports further functional genomic study of this species.