Human iPSC Culture: Standard Operating Procedure v1

To ensure consistent and high-quality maintenance of induced pluripotent stem cells (iPSCs) by implementing standardized protocols and adhering to good cell culture practices. This includes minimizing inter- and intra-batch variability, preventing microbial and cross-contamination, and optimizing culture conditions such as media composition, passaging frequency, and confluency thresholds to support reproducible and robust downstream applications including differentiation, gene editing, and transcriptomic profiling.