Preliminary Study on Cryopreservation of Local Chicken Spermatozoa in Liquid Nitrogen

This research was designed to investigate the most effective cryoprotectant to be used for spermatozoa of local chicken Gallus gallus domesticus in liquid nitrogen. Semen was collected from local chickens through abdominal massaging. The sperm motility and concentration of fresh semen were immediately evaluated, with subsequent weekly evaluation of the cryopreserved semen in liquid nitrogen for a maximum of three weeks. The cryoprotecting facilities used were dimethyl sulfoxide and phosphate buffered saline (DP); dimethyl sulfoxide and Ginzburg fish ringer (DF); glycerol and phosphate buffered saline (GP); glycerol and Ginzburg fish ringer (GF); egg yolk and phosphate buffered saline (EY+P); egg yolk and Ginzburg fish ringer (EY+F); quail Egg yolk and phosphate buffered saline (QEY+P); quail egg yolk and Ginzburg fish ringer (QEY+F). Results showed that the motility and concentration of freshly collected sperm recorded 70% and 1.79×109 spermatozoa/ml, respectively, while there was a decrease in sperm motility and concentration over time. Importantly, the yolk-based cryoprotective agents with Ginzburg fish ringer had the most effective cryoprotective properties; however, the quail egg yolk was better and could be employed for long-term cryopreservation.