Integrating optical coherence tomography and bioluminescence with predictive modeling for quantitative assessment of methicillin-resistant S. aureus biofilms
Bioluminescence alone underestimates bacterial burden in dense, metabolically suppressed MRSA biofilms. The combination of BLI with OCT-derived structural metrics enables accurate, nondestructive quantification of viable bacterial load. This approach provides a robust toolset for preclinical evaluation of antimicrobial therapies, particularly for optimizing PDT dosimetry and assessing biofilm response in translational infection models.
Authors
- Valentin Demidov (ORCID: https://orcid.org/0000-0002-0960-3858)
- Ida Leah Gitajn (ORCID: https://orcid.org/0000-0001-8649-7385)
- Jonathan T. Elliott (ORCID: https://orcid.org/0000-0002-8485-0234)
- Natalia Demidova (ORCID: https://orcid.org/0000-0002-6048-5316)
- Jason R. Gunn
- Olivia P. Jackson
Institutions
- Dartmouth College (US)
- Dartmouth–Hitchcock Medical Center (US)
Publication Details
- Journal
- Journal of Biomedical Optics
- Published
- 2025-09-23
- DOI
- https://doi.org/10.1117/1.jbo.30.s3.s34111
- Citations
- 2
- Primary Topic
- Optical Coherence Tomography Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.35
Funders
- Dartmouth College
- Dartmouth Cancer Center
- National Institutes of Health