Receptor gene expressions and their association with biochemical parameters in primary hyperparathyroidism patients

Aims: Primary hyperparathyroidism (PHPT) is recognized by excessive parathyroid hormone (PTH) secretion, leading to hypercalcemia and systemic complications. This study aimed to investigate the gene expression profiles of PTH, calcium-sensing receptor (CaSR), vitamin D receptor (VDR), fibroblast growth factor receptor 1 (FGFR1), and α-Klotho from parathyroid adenomas among PHPT patients and to explore their correlations with biochemical parameters. Methods: Parathyroid adenoma samples were obtained from 20 PHPT patients who underwent parathyroidectomy. RNA was isolated, and gene expression levels were quantified using one-step reverse transcription quantitative PCR (RT-qPCR). Fold change values were calculated utilizing gene-specific patient average ΔCt values as references. Biochemical data, including serum calcium, phosphorus, PTH, and vitamin D (25-OH), as well as gender, age, and adenoma volumes, were statistically analyzed for correlation with gene expression patterns. Results: Gene expression analysis revealed significantly lower CaSR expression in small adenomas (