Acute Oak Decline Pathogens in Urban Spaces: An Occurrence Analysis Based on the Example of Wrocław, Poland

Acute Oak Decline (AOD) is a progressive disease affecting oaks across Europe and is increasingly recognised as a threat to the health of forests and urban trees. While the occurrence of this disease has been documented in forest ecosystems, its presence in urban landscapes is still poorly understood. In this study, the occurrence of AOD-associated bacteria (Brenneria goodwinii, Gibbsiella quercinecans, Rahnella victoriana, Lonsdalea quercina) was investigated in Quercus robur and Q. rubra growing in urban areas of Wrocław, Poland. Multiplex real-time PCR analyses confirmed the pathogens in 11 trees, with B. goodwinii being the most common species. Importantly, we provide the first confirmed detection of B. goodwinii in Q. rubra under urban conditions, possibly the first such detection in Europe. The results show the occurrence of AOD-associated pathogens in urban environments, suggesting that such habitats may provide favourable conditions for their occurrence. However, further investigations, including epidemiological and spatial analyses, are needed to clarify whether urban areas contribute to the persistence or spread of these pathogens. Beyond local documentation, our results emphasise the need to include urban ecosystems in AOD surveillance and highlight potential pathways for pathogen adaptation and spread in cities. This work provides new insights into the ecology of AOD in anthropogenically modified habitats and has direct implications for urban tree health monitoring, biodiversity conservation, and the development of integrated management strategies.