Protocol for Hi-C-based identification of chromosomal inversions in mosquitoes
Chromosomal inversions play an important role in the genomic evolution and adaptation of mosquito species. Here, we present a protocol for detecting chromosomal inversions in mosquitoes using Hi-C technology and chromatin contact heatmaps. We describe the steps for Hi-C library preparation using the Hi-C Arima+ kit, along with the Arima Library Prep Module, and provide several adaptations for mosquito samples. We then outline procedures for Hi-C data analysis, Hi-C heatmap generation, and the identification of polymorphic and fixed inversions. For additional details on the use and execution of this protocol, please refer to Lukyanchikova et al.<sup>1</sup>.
Authors
- Varvara Lukyanchikova (ORCID: https://orcid.org/0000-0002-1828-4721)
- Maria V. Sharakhova (ORCID: https://orcid.org/0000-0002-5790-3548)
- Dmitry A. Karagodin (ORCID: https://orcid.org/0000-0001-6413-7928)
- Igor V. Sharakhov (ORCID: https://orcid.org/0000-0003-0752-3747)
- Ilya I. Brusentsov (ORCID: https://orcid.org/0000-0003-3104-2441)
Institutions
- National Research Tomsk State University (RU)
- Institute of Cytology and Genetics (RU)
- Virginia Tech (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-09-23
- DOI
- https://doi.org/10.1016/j.xpro.2025.104087
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 0.00