Protocol for monitoring the growth of Solanum betaceum non-embryogenic callus using electrochemical impedance spectroscopy

Callus cultures have important biotechnological applications. Here, we describe the use of electrochemical impedance spectroscopy (EIS) as a real-time and non-destructive approach to monitor callus growth. We describe the steps to obtain in vitro tamarillo clones from seeds and from these non-embryogenic calluses. We detail the steps for the preparation of the EIS apparatus and experiment and a parallel growth assay. We also provide complementary microscopy assays to complement the EIS experiment and outline the main data treatment steps. For complete details on the use and execution of this protocol, please refer to Caeiro et al.¹.