Protocol to set up the immuno-microfluidic system for rapid measurement of salivary cortisol level

Microfluidics has versatile applications in the field of biosensors. We present a protocol for assembling a microfluidic system to rapidly measure cortisol in saliva. This protocol prepares microfibrous reactors immobilized with antibodies as an alternative to a 96-well plate and integrates a flow system, reactor platform, and detection device. The output current signal directly reflects cortisol levels for rapid evaluation. For complete details on the use and execution of this protocol, please refer to Wang et al.¹.