Figure 4 from Preclinical Evaluation of Folate Receptor-α Chimeric Antigen Receptor T Cells Exhibits Highly Efficient Antitumor Activity against Osteosarcoma

<p><i>In vivo</i> functional evaluation of FH FOLR1-CAR T cells in a metastatic U-2 OS xenograft model. <b>A,</b> Average radiance of pulmonary osteosarcoma disease measured over 180 days from tumor inoculation by tail vein in mice treated with FH FOLR1-CART vs. NT T cells. <b>B,</b> Kaplan–Meier survival curves of xenografts treated with NT or FH FOLR1-CAR T cells. <i>n</i> = 3 per group. Statistical difference in survival evaluated using Mantel–Cox log-rank test. <b>C,</b> Biolumiscence imaging of metastatic pulmonary U-2 OS tumor injected by tail vein in mice treated with NT or FH FOLR1-CAR T cells. Radiance scale indicates progressive metastatic osteosarcoma from blue to red. *Day 13 images use a minimum radiance cutoff of 5e4 p/second/cm<sup>2</sup>/sr to show the presence of tumor engraftment prior to treatment. A minimum radiance cutoff of 2e5 p/second/cm<sup>2</sup>/sr was used for posttreatment images to avoid noise created by excess radiance detected. X indicates death. D, day. <b>D</b> and <b>E,</b> Axial noncontrast CT scan in lung windows in two different mice. <b>D,</b> Lobulated soft tissue density (solid arrow) with spiculated margins is present in the right upper lung of mouse treated with NT T cells at 133 days after osteosarcoma injection by tail vein. <b>E,</b> Note the normal appearance of the right upper lung, with normal lung markings and the absence of soft tissue mass (open arrow), of mouse treated with FH FOLR1-CAR T cells at same time point. No other lesions were present within the remainder of the lungs. <b>F,</b> H&E stain of lung tissue harvested from mouse treated with FH FOLR1-CART at 5× magnification. No tumors or any sign of disease observed within sections taken. <b>G,</b> H&E stain of lung tissue harvested from mouse treated with NT T cells at 5× magnification. Expanding and effacing the normal lung are numerous poorly circumscribed, invasive, moderately cellular proliferation of neoplastic mesenchymal cells arranged in trabeculae and streams. Neoplastic cells occasionally surround eosinophilic osteoid and islands of the basophilic cartilaginous matrix.</p>