Whole genome study and construction of SHERLOCK detection method for endemic strains of <i>Burkholderia pseudomallei</i> in Hainan based on third-generation sequencing

Melioidosis is a highly pathogenic infectious disease caused by a gram-negative bacterium of <i>Burkholderia pseudomallei</i> (<i>Bp</i>). The traditional gold standard for diagnosing melioidosis is still isolation and culture from clinical samples. Although this method has high specificity, it has low sensitivity and is time-consuming, which often leads to misdiagnosis or missed diagnosis of melioidosis, affecting subsequent treatment. In this study, recombinase polymerase amplification technology and clustered regularly interspaced short palindromic repeats/Cas13a technology were combined to establish the Specific High-sensitivity Enzymatic Reporter Unlocking detection technology, which can achieve rapid and accurate identification of <i>Bp</i>, providing a new method for the early diagnosis of melioidosis.