RB-TnSeq elucidates dicarboxylic-acid-specific catabolism in β-proteobacteria for improved plastic monomer upcycling
Upcycling the carbon in plastic wastes to value-added products is a promising approach to address the plastic waste and climate crises, and dicarboxylic acid metabolism is an important facet of several approaches. Improving our understanding of the genetic basis of this metabolism has the potential to uncover new enzymes and genetic parts for engineered pathways involving dicarboxylic acids. Our data set is the most comprehensive interrogation of dicarboxylic acid catabolism to date, and this work will be of utility to researchers interested in both plastics bioproduction and upcycling applications.
Authors
- Jay D. Keasling (ORCID: https://orcid.org/0000-0003-4170-6088)
- Jacob B. Roberts (ORCID: https://orcid.org/0000-0001-7564-1708)
- Matthew R. Incha (ORCID: https://orcid.org/0000-0003-4122-3342)
- Aindrila Mukhopadhyay (ORCID: https://orcid.org/0000-0002-6513-7425)
- Matthias Schmidt (ORCID: https://orcid.org/0000-0002-0161-8326)
- Julie Lynch
- Allison N. Pearson (ORCID: https://orcid.org/0000-0002-5383-4944)
- Graham A. Hudson (ORCID: https://orcid.org/0000-0002-3715-4279)
- Adam M. Deutschbauer (ORCID: https://orcid.org/0000-0003-2728-7622)
- Mitchell G. Thompson
- Patrick M. Shih (ORCID: https://orcid.org/0000-0002-2119-3345)
- Javier Menasalvas (ORCID: https://orcid.org/0000-0002-4003-4999)
- Aaron A. Vilchez (ORCID: https://orcid.org/0009-0005-3847-189X)
- Cindy Ho (ORCID: https://orcid.org/0009-0008-3067-1004)
Institutions
- QB3 (US)
- Lawrence Berkeley National Laboratory (US)
- Novo Nordisk Foundation (DK)
- Quantitative BioSciences (US)
- Innovative Genomics Institute (US)
- Joint BioEnergy Institute (US)
- RWTH Aachen University (DE)
- University of California, Berkeley (US)
- Technical University of Denmark (DK)
Publication Details
- Journal
- Applied and Environmental Microbiology
- Published
- 2025-09-22
- DOI
- https://doi.org/10.1128/aem.00924-25
- Citations
- 1
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.81