Simultaneous 4G and 5G EMF Exposure and Field Uniformity in a Reverberation Chamber for Animal Studies

The design and validation of a reverberation chamber (RC) specifically constructed for conducting large-scale experimental animal carcinogenicity studies using RF electromagnetic fields (EMF) relevant to contemporary 4G and 5G mobile communication (900 MHz, 2.12 GHz, and 3.65 GHz) is proposed. The RC’s electric field (E-field) uniformity is evaluated under four practical loading conditions: empty, apparatus only, and two apparatus variations with 80 experimental animals (Sprague–Dawley rats) with approximate weights 400 g and 520 g, respectively. Measurement results show E-field uniformity better than 1.36 dB under all test conditions, with frequency-dependent variation becoming negligible once the RC is loaded with cage racks and 80 rats. Additionally, a predictive method is introduced to estimate composite E-field intensities under simultaneous multi-frequency exposures, potentially reducing experimental measurements. These findings confirm that the designed RC is capable of accurately evaluating RF EMF exposure in biological studies.