The PAS domain of the polarly localized histidine kinase FlrB in <i>Vibrio cholerae</i> controls class III flagellar transcription and contributes to intestinal colonization
<i>Vibrio cholerae</i> causes the severe diarrheal disease cholera when it colonizes the human intestine. The bacteria are able to swim due to a polar flagellum, and motility is linked to disease, as well as environmental persistence. This study demonstrates that FlrB, a key regulatory protein, localizes to the cell pole and controls flagellar gene transcription via a PAS domain that regulates autophosphorylation. The ability of FlrB to switch between active and inactive forms is critical for motility, as well as intestinal colonization, emphasizing the importance of <i>V. cholerae</i> swimming for its ability to cause disease.
Authors
- Igor B. Zhulin (ORCID: https://orcid.org/0000-0002-6708-5323)
- Hong Cai (ORCID: https://orcid.org/0000-0001-9699-7260)
- K.J.F. Satchell (ORCID: https://orcid.org/0000-0003-3274-7611)
- Mark Eppinger (ORCID: https://orcid.org/0000-0001-5267-5219)
- Jesús A. Romo (ORCID: https://orcid.org/0000-0002-1683-2288)
- Karl E. Klose (ORCID: https://orcid.org/0000-0003-2007-1407)
- Jiawei Xing (ORCID: https://orcid.org/0000-0002-7691-1180)
- G. Minasov (ORCID: https://orcid.org/0000-0001-5460-3462)
- Barb Himes
- Nicole L. Inniss (ORCID: https://orcid.org/0000-0002-1510-7412)
- Adrian Mejia-Santana (ORCID: https://orcid.org/0000-0002-4206-1384)
- Yufeng Wang (ORCID: https://orcid.org/0000-0002-5819-0578)
- Venus Stanton (ORCID: https://orcid.org/0009-0003-9567-9051)
Institutions
- Northwestern University (US)
- Texas Center for Infectious Disease (US)
- The Ohio State University (US)
Publication Details
- Journal
- mBio
- Published
- 2025-09-22
- DOI
- https://doi.org/10.1128/mbio.02379-25
- Citations
- 1
- Primary Topic
- Vibrio bacteria research studies
- Type
- article
- Field-Weighted Citation Impact
- 1.80