Whole‐genome sequencing reveals novel ryanodine receptor mutations <scp>P4568L</scp> and <scp>Y4701D</scp> conferring high‐level chlorantraniliprole resistance in <scp> <i>Spodoptera litura</i> </scp> (Lepidoptera: Noctuidae)

This research elucidates the genetic mechanisms underlying chlorantraniliprole resistance in S. litura and provides the first evidence of the Y4701D mutation conferring diamide resistance in Noctuidae. These findings enable the development of rapid molecular diagnostic assays which are vital for proactive resistance management. Given that the resistance is recessive in nature it can remain undetected by simple conventional bioassays until it becomes widespread. The findings significantly advance the field of resistance management by facilitating timely interventions to prevent resistance development, thereby supporting more effective integrated pest management. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.