Combined Weight Loss and Exercise Training Alters Skeletal Muscle Subcellular Lipid Localization and Intermuscular Adipose Tissue Cellular Composition
Evaluation of subcellular fractionated muscle revealed decreases in sarcolemmal and nuclear triglycerides and increases in nuclear diacylglycerols and cytosolic sphingolipids postintervention. Weight loss revealed alteration in the cellular composition of intermuscular adipose tissue and upregulation of genes related to muscle remodeling and cellular respiration. These findings dissociate improvements in insulin sensitivity from total muscle 1,2-diacylglycerol and sphingolipid levels and highlight roles of intermuscular adipose tissue remodeling for enhanced insulin sensitivity.
Authors
- Lauren M. Sparks (ORCID: https://orcid.org/0000-0002-9257-5666)
- Purevsuren Jambal
- Leigh Perreault (ORCID: https://orcid.org/0000-0002-1481-7535)
- Christy M Nguyen (ORCID: https://orcid.org/0000-0002-3897-6207)
- Anna A. Kerege
- Emily Macias
- Marcus Seldin (ORCID: https://orcid.org/0000-0001-8026-4759)
- Katie L. Whytock (ORCID: https://orcid.org/0000-0003-3486-9452)
- Karin A. Zemski Berry (ORCID: https://orcid.org/0000-0002-7089-691X)
- Martin J. Walsh (ORCID: https://orcid.org/0000-0001-8339-8285)
- Christopher D. Johnson (ORCID: https://orcid.org/0000-0002-2972-3072)
- Darcy Kahn (ORCID: https://orcid.org/0000-0002-3764-1202)
- Amanda Garfield (ORCID: https://orcid.org/0009-0006-1449-4939)
- Bryan C. Bergman (ORCID: https://orcid.org/0000-0002-9561-1052)
- Simona Zarini (ORCID: https://orcid.org/0000-0002-7460-3299)
- Simon Bowen (ORCID: https://orcid.org/0000-0001-9343-9837)
- Carlos H Viesi
- Yifei Sun (ORCID: https://orcid.org/0000-0002-5530-8833)
- Ian Tamburini
- Tyler Stepaniak
Institutions
- Orlando Health (US)
- University of California, Irvine (US)
- Translational Research Institute for Metabolism and Diabetes (US)
- Irvine University (US)
- University of Colorado Anschutz Medical Campus (US)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- Diabetes
- Published
- 2025-09-22
- DOI
- https://doi.org/10.2337/db25-0492
- Primary Topic
- Adipose Tissue and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00