Aniline Blue as a Chromogenic Reagent for Spectrophotometric Determination of Trifluoperazine Hydrochloride

Background: Trifluoperazine hydrochloride is a group 3 phenothiazine antipsychotic used primarily to treat schizophrenia and psychotic disorders by rebalancing brain chemicals and reducing symptoms like anxiety and hallucinations . Methods: A novel method for determining of trifluoperazine hydrochloride has been established in an acidic medium using aniline blue dye. Results: A linear decrease in dye absorbance was observed with increasing drug concentration as a result of electrostatic attraction in ion association formation. The effects of various experimental parameters such as solution pH, time, temperature ,and dye concentration on decreasing the absorbance of the blue dye were investigated in detail at λmax 592 nm, and a good linear relationship was obtained between ΔA and the concentration of trifluoperazine hydrochloride in the range of 0.5 -7.0 μg/mL with a determination coefficient of 0.9962, recovery average % of 97.80% ,Limit of detection(LOD) and limit of quantification(LOQ) values are 0.3319 ㎍.ml-1and 1.1065 ㎍.ml-1 respectively. The relative Error RE% of -1.2 to -3.2% and a relative standard deviation RSD % of 0.35 to 2.79%. Conclusion: This proposed method had been successfully applied to determine of trifluoperazine hydrochloride in tablet form accurately and precisely.