Neural network reconstruction and motor function recovery in traumatic brain injury rat models via a 3D collagen/heparan/bFGF/NGF scaffold combined with mesenchymal stem cells COMBINED SCAFFOLD-MSC THERAPY FOR TBI REPAIR

<i>Objective.</i>To address the limited innate regenerative capacity of neural tissues following traumatic brain injury (TBI) by developing a novel therapeutic intervention.<i>Approach.</i>We engineered a composite scaffold using 3D bioprinting to integrate mesenchymal stem cells (MSCs) with collagen-heparan matrices supplemented with basic fibroblast growth factor (bFGF) and nerve growth factor (NGF), creating a 3D-CH-bFGF/NGF-MSCs construct.<i>Main results.</i>The engineered construct demonstrated favorable biomechanical characteristics and cytocompatibility. In rat TBI models, this intervention significantly enhanced cognitive recovery and sustained sensorimotor function restoration. Histopathological analyses revealed corresponding neural network regeneration through axonal regrowth, synaptogenesis reinforcement, and myelination enhancement at injury sites.<i>Significance</i>. This study demonstrates the therapeutic potential of a 3D-bioprinted, growth factor-enhanced MSC-scaffold construct to promote structural and functional neural repair after TBI, offering a promising strategy for neural tissue regeneration.