Evaluation of Antimicrobial Activity of <i>Aloe vera</i> Gel Extract on Hospital‐Acquired Methicillin‐Resistant <i>Staphylococcus aureus</i>

<b>Introduction:</b> <i>Aloe vera</i> has a long history as a medicinal plant with numerous therapeutic uses. Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a type of bacteria resistant to commonly used antibiotics such as penicillin. Hospital-acquired methicillin-resistant <i>Staphylococcus aureus</i> (Ha-MRSA) refers to MRSA infections that arise in healthcare settings, usually 48 h or longer after hospital admission or in individuals with recent hospitalization. This research is aimed at investigating the effectiveness of <i>Aloe vera</i> gel extract against Ha-MRSA. <b>Methods:</b> The agar well boring diffusion and the broth dilution test methods were employed, respectively, to assess the susceptibility of Ha-MRSA to <i>Aloe vera</i> gel extract in ethanol and DMSO medium. These extraction media were compared for their effectiveness as the solvent of extraction for the susceptibility test. <b>Results:</b> A minimum inhibitory concentration of 0.2 mg/L of <i>Aloe vera</i> gel extract in the ethanolic extraction medium was achieved, indicating susceptibility of the Ha-MRSA strain as recommended by EUCAST/ESCMID. <b>Conclusion:</b> <i>Aloe vera</i> gel ethanol extract was sensitive against Ha-MRSA strains but was resistant in the DMSO extraction method. It is recommended that further research be carried out to ascertain the bioactive compounds responsible for antimicrobial activity.