ENDOCRINE FACTORS ASSOCIATED WITH SUCCESSFUL IN VITRO FERTILIZATION PREGNANCY

Background: Infertility is the term used when a clinical pregnancy cannot be achieved within at least 12 months. Thyroid dysfunction impacts the anti-Mullerian hormone (AMH), a recognized marker of ovarian function. Proper growth, sexual maturation, and reproductive processes rely on gonadotropins, peptide hormones that regulate ovarian and testicular functions. Objective: The objective of this study is to assess the serum levels of specific endocrine factors, including anti-mullerian hormone, prolactin, gonadotropins, sex-related hormones, and thyroid-stimulating hormones in women undergoing IVF treatment. Materials and Methods: The study consisted of women who underwent IVF at the Australian Concept Infertility Medical Center, Lahore. This was a cross-sectional study involving 80 women undergoing IVF. The causes of infertility were distributed as follows: female infertility, primary and secondary, tubal factor infertility, polycystic ovary syndrome, endometriosis, and other reasons. Blood samples were collected from patients undergoing IVF on various days to analyze endocrine variables. Enzyme-linked immunosorbent assay (ELISA) kits were used to detect FSH, LH, estrogen, progesterone, AMH, TSH, and prolactin. Statistical analysis was conducted using SPSS. Results: Mean age of women was 33.42±4.74 years. Among women 76(95%) were nulliparous and only 3(3.8%) women were prim-parous while only 1(1.3%) women present with parity 4. Mean body mass index of women was 27.20±4.68. 64(80%) women were present with primary and 16(20%) presents with secondary infertility. The most frequent blood group among women was O+ve (57.5%), followed by B+ve (31.25%), AB+ve (6.25%), and A+ve (5%), respectively. Mean FSH level in women was 6.36±2.92. Mean LH level in women was 5.24±2.63. Mean prolactin level in women was 19.71±12.33. Mean serum AMH level among women was 2.49±1.68. The mean AMH level ranges between 0.09 and 19.30. Mean TSH level among women was 2.02±1.29. Mean estrogen and progesterone levels among women were 108.70±80.44 and 2.98±2.19, respectively. Conclusion: Our findings demonstrated that FSH, LH, prolactin, AMH, and estrogen levels were slightly higher among women with positive pregnancy outcomes. Women with negative pregnancy outcomes had higher values for serum TSH and progesterone levels.