The use of hexanucleotide SSR-markers for molecular genetic certification of Cannabis sativa L.

This research addresses the urgent issue of molecular genetic certification of hemp ( Cannabis sativa L.) varieties, crucial amid rapid breeding progress and expanding industrial use. The aim of the research is a development a complex of SSR markers that will ensure reliable differentiation of hemp varieties for breeding and seed production practices and molecular genetic certification. The work presents the results of the analysis of twelve varieties of monoecious industrial hemp of the Central Russian ecotype using two sets of SSR markers: highly polymorphic dinucleotide markers and developed hexanucleotide microsatellite markers. The study included DNA extraction, PCR amplification, allele detection and statistical data processing. The results obtained showed an extremely high level of intravarietal polymorphism when using highly polymorphic markers, which complicates their use for genetic certification. The developed complex of 10 hexanucleotide SSR markers covering all chromosomes of the genome made it possible to carry out intravarietal differentiation of samples.