Quantitative Analysis of Glutathione Utilizing Highly Luminescent Gold Nanoclusters
Glutathione (GSH), a pivotal regulator of cellular redox homeostasis, requires precise monitoring for clinical diagnostics. This work develops bovine serum albumin-templated gold nanoclusters (BSA-AuNCs) through in situ chloroauric acid reduction, exhibiting orange fluorescence (λ<sub>em</sub> = 610 nm) with 35.8% quantum yield. Systematic evaluation of pH-dependent and time-resolved quenching behavior revealed optimal GSH detection at physiological pH 7.4 within 3 min. The nanozyme demonstrated linear responsivity from 10 nM to 3 μM (Limit of Detection, LOD = 8.7 nM) and validated applicability in biological matrices (urine/serum) with 98.0%-103.3% recovery rates. This photoluminescent platform enables reliable GSH biomarker quantification, advancing point-of-care testing for oxidative stress disorders.
Authors
- Jibin Song (ORCID: https://orcid.org/0000-0003-4771-5006)
- Soh Fong Lim (ORCID: https://orcid.org/0000-0003-1768-3971)
- Hongyu Mou
- Wen He (ORCID: https://orcid.org/0000-0001-6119-971X)
- Shukai Wu
- Ling Mo
- Yanping Qu (ORCID: https://orcid.org/0009-0004-7101-4626)
Institutions
- Qilu University of Technology (CN)
- Universiti Malaysia Sarawak (MY)
- Beijing University of Chemical Technology (CN)
Publication Details
- Journal
- Journal of Biomedical Materials Research Part A
- Published
- 2025-09-22
- DOI
- https://doi.org/10.1002/jbm.a.37988
- Primary Topic
- Nanocluster Synthesis and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00