Effectiveness of Cream Containing Medicinal Plants in the Treatment of Second Degree Burns: Biochemical and Histopathological Investigation

Burn injuries represent severe pathophysiological conditions characterized by increased oxidative stress and impaired tissue integrity, necessitating effective therapeutic interventions. This study evaluates the biochemical and histopathological effects of a novel cream formulation containing Juglans regia leaf extract, Argania spinosa kernel oil, Prunus dulcis oil, and Lavandula angustifolia oil on second-degree burn wounds. Using a Wistar-albino rat model, oxidative stress markers, including superoxide dismutase (SOD) and malondialdehyde (MDA), were analyzed alongside histopathological assessments to determine the formulation’s therapeutic potential. The results demonstrated a significant increase in SOD levels and a concurrent reduction in MDA levels in the treatment group, suggesting an enhanced antioxidant response and reduced lipid peroxidation. Histopathological evaluations revealed accelerated epithelialization, increased fibroblast activity, and improved keratinization compared to the burn control and silver sulfadiazine groups. These findings indicate that the tested formulation effectively mitigates oxidative damage while promoting tissue regeneration. As a potential alternative to conventional burn treatments, this plant-based formulation may offer an innovative and cost-effective therapeutic strategy for managing second-degree burns. However, further clinical studies are required to confirm its efficacy in human applications.