Salivary Biomarker Analysis to Distinguish Between Health and Periodontitis Status: A Preliminary Study

<b>Objective:</b> This study aims to explore the feasibility of a non-invasive and simple method for discriminating between health and periodontitis (PRD), facilitating early and objective diagnosis of PRD before detectable periodontal attachment loss and monitoring treatment outcomes. <b>Methods:</b> Salivary samples were collected from 16 PRD-free patients (G1) and 10 patients with PRD (G2). The analysis included salivary matrix metalloproteinase-8 (MMP-8), major anti-inflammatory interleukins (IL-4 and IL-10), pro-inflammatory cytokines (IL-1β, IL-8, and interferon α [IFN-α]), and the cytokine IL-6. Clinical and salivary assessments were performed at baseline (TP0) for both groups and after periodontal treatment for G2 (TP1). <b>Results:</b> PRD indices were significantly higher in G2-TP0, lower in G1, and intermediate in G2-TP1. Except for IL-6, the biomarkers were significantly correlated with nearly all PRD clinical indices. Logistic regression and receiver operating characteristic (ROC) curve analyses showed statistical significance for MMP-8, IL-1β, IL-4, IL-8, and IL-10 when comparing G1 and G2 at TP0. MMP-8 was also significant when comparing G2-TP0 and G2-TP1, while IL-1β and IL-10 showed borderline significance. IL-8 was significant when comparing G1 and G2-TP1. <b>Conclusions:</b> The molecular network demonstrated great potential for early diagnosis and monitoring of therapy response, providing a promising basis for future research. Among the biomarkers, MMP-8, IL-1β, IL-4, IL-8, and IL-10 showed the strongest statistical correlations with the clinical indices. The inflammation-related biomolecules behaved differently among untreated PRD (G2-TP0), treated (G2-TP1), and healthy individuals (G1). Healthy individuals and those with treated PRD may regulate inflammation significantly differently from those with untreated PRD.