Protocol to assess the cytotoxicity of autologous human skin immune cells against senescent fibroblasts

Immune clearance of senescent cells contributes to their control in aging tissues; however, it remains unclear how immunity against senescent cells is regulated in humans. Here, we provide a protocol to measure the cytotoxicity of autologous human skin immune cells against senescent dermal fibroblasts. We describe the ex vivo co-culture experiments using normal and senescent fibroblasts together with autologous immune cells isolated from the human skin. This protocol is applicable to understanding the mechanism of immune surveillance and clearance of senescent cells in humans. For complete details on the use and execution of this protocol, please refer to Hasegawa et al.¹.