Multi-functional poly(N-isopropylacrylamide)-based hydrogel with hemostasis, antibacterial, and sustained drug release properties for infected wound treatment

Wound infections impede healing, exacerbate discomfort, and risk severe complications. Therefore, it is urgent to develop an innovative wound dressing that combines hemostasis, antibacterial and wound healing. This study presented a composite multifunctional hydrogel dressing (GA-CP@LVN) based on Poly(N-isopropylacrylamide) (PNIPAM), chitosan (CS), silver nanoparticles-modified graphene oxide (GO-Ag NPs), and levofloxacin (LVN). In vitro and in vivo studies demonstrated GA-CP@LVN's biocompatibility, photothermal properties, high LVN encapsulation efficiency, and sustained release. Moreover, GA-CP@LVN exhibited superior antibacterial and hemostatic functionalities. Notably, GA-CP@LVN showed potent synergy in hemostasis, bacteriostasis, and cell proliferation, significantly enhancing wound healing in S. aureus-infected murine models. Thus, GA-CP@LVN emerges as a promising novel dressing for infected wound repair.