Cytotoxicity and Wound Healing Effect of Camphor Menthol Based Natural Deep Eutectic Solvents Against Human Epidermoid Carcinoma (A431) Cell Line

Introduction: Skin cancer is a serious health issue and finding effective treatments is crucial. This study investigated the effects of camphor-menthol based natural deep eutectic solvent on human epidermoid carcinoma A431-cells, a model for skin cancer. Method: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to assess cell viability and cytotoxicity by measuring absorbance at 570 nm. The absorbance at 570 nm was measured to determine the optical density values, and cell viability was calculated as a percentage. Additionally, an in vitro scratch assay was performed to evaluate the impact of camphor-menthol based natural deep eutectic solvent on cell migration. Results: The results revealed a concentration-dependent decrease in both optical density values and cell viability, with an inhibitory concentration of 311.7 µg/ml. The treated group exhibited a significant reduction in wound area compared to the control, suggesting potential antimigratory effects. Since cell migration is key to cancer spread, this could be an important finding. Conclusion: In conclusion, these findings highlight the cytotoxic and migration-inhibiting properties of camphor-menthol based natural deep eutectic solvent, supporting its potential as a therapeutic agent against skin cancer. These findings highlight the potential of natural compounds in cancer treatment. More research is needed to understand how camphor-menthol based natural deep eutectic solvent works and to confirm these results in more complex biological systems.